Journal: Cancers

Article Title: A Simple Three-Dimensional In Vitro Culture Mimicking the In Vivo-Like Cell Behavior of Bladder Patient-Derived Xenograft Models

doi: 10.3390/cancers12051304

Figure Lengend Snippet: Fluorescence confocal microscopy images of PDX tumor spheroids BL0293 ( A , C ) and BL0808 ( B , D ). Cell nuclei stained in blue, hypoxic inducible factor 1α (HIF-1α) and Ki67 protein stained in green; 20× magnification, scale bar = 100 μm.

Article Snippet: The spheroids were stained with 1 μg/mL of Hoechst Stain 33342 (Cell Signaling Technology, Danvers, MA, USA), 1:250 anti-human hypoxic inducible factor (HIF) 1α (BD Transduction Laboratories TM ), and 1:250 anti-human Ki67 8D5 (Cell Signaling Technology, Danvers, MA, USA).

Techniques: Fluorescence, Confocal Microscopy, Staining

Journal: Journal of Translational Medicine

Article Title: Hypoxia directed migration of human naïve monocytes is associated with an attenuation of cytokine release: indications for a key role of CCL26

doi: 10.1186/s12967-020-02567-7

Figure Lengend Snippet: Proteome profiling arrays. a Representative array membranes detecting 31 cytokines involved in chemotaxis. The top 5 secreted cytokines are presented in b while the top 5 hypoxia regulated cytokines are shown in c . For a detailed description of the array proteins please refer to Additional file : Figure S2

Article Snippet: Analyses of the cytokines and chemokines secreted by monocytes under hypoxic and normoxic conditions were performed using Proteome Profiler Human Chemokine Arrays (ARY017, R&D Systems) according to the manufacturer`s protocol provided with the assay kit.

Techniques: Chemotaxis Assay

Journal: Journal of Bone and Mineral Research

Article Title: Growth Hormone Regulates the Balance Between Bone Formation and Bone Marrow Adiposity

doi: 10.1359/jbmr.091015

Figure Lengend Snippet: Effect of treatment with 17β-estradiol (E 2 ), insulin-like growth factor 1 (IGF-1), or growth hormone (GH) on uterine weight ( A ) and bone marrow adipocyte area/tissue area in the proximal tibia ( B ) in sexually mature hypophysectomized (HYPOX) female rats (experiment 3). Hormone replacement was started 10 days following HYPOX. Values are mean ± SE; n = 8 per group). a Significantly different from control, p < .01.

Article Snippet: The rats were delivered to our facility overnight on postoperative day 7 and were divided into the following treatment groups: (1) HYPOX + VEH (saline, n = 8), (2) HYPOX + E 2 (4.8 μg/kg 17β-estradiol, Sigma, via daily sc injection, n = 13), (3) HYPOX + GH (800 μg/kg/day recombinant human GH, Genentech, via three daily sc injections, n = 8), and (4) HYPOX + IGF-1 (long R3, 200 μg/kg/day, GroPep, Adelaide, Australia, via once-daily sc injection, n = 5).

Techniques: Control